Neonatal hyperoxia induces sex-dependent pulmonary cellular and transcriptomic changes in an experimental mouse model of bronchopulmonary dysplasia

To investigate sex-dependent molecular and cellular programming involved in hyperoxia, we surveyed the mouse lung using single cell RNA sequencing (scRNA-seq). Overall design: WT C57BL/6 (Charles River) mice were used in the study. For hyperoxia, pups with dam were placed in cages in a hyperoxia chamber for 14 days from P1 to P14. We used 3 females and 3 males/experimental condition (room air and hyperoxia) for scRNA-seq.