Dyrk1a dosage effects on the transcriptome of embryonic hearts from the Dp1Tyb mouse model of Down syndrome using scRNAseq

We performed single cell RNAseq on whole hearts from E13.5 Dp1Tyb embryos and from wild-type littermates, as well as hearts from E13.5 Dp1TybDyrk1a+/+/- embryos and wild-type littermates. We identified 14 clusters which were assigned to individual cell types based on expression of marker genes. Single cell RNAseq analysis of 1 E13.5 Dp1Tyb heart, 3 Dp1TybDyrk1a+/+/- embryonic hearts and 3 wild-type embryonic hearts.本研究对胚胎发育第13.5天（E13.5）的Dp1Tyb胚胎及其野生型同窝胚胎的完整心脏，以及胚胎发育第13.5天的Dp1TybDyrk1a+/+/-胚胎及其野生型同窝胚胎的心脏开展了单细胞RNA测序（single cell RNAseq）。我们共鉴定出14个细胞簇，并基于标记基因的表达特征将其注释为相应的细胞类型。本次实验共对1例胚胎发育第13.5天的Dp1Tyb胚胎心脏、3例Dp1TybDyrk1a+/+/-胚胎心脏以及3例野生型胚胎心脏进行了单细胞RNA测序分析。