Gene expression profiling of Foxr2-induced brain tumors

The goal of this study is profile gene expression patterns of Foxr2-induced brain tumor cells. Foxr2 overexpression in the brain induced tumor formation in the olfactory bulb (OB) and the brainstem (BS) under the p53 deficient background. Tumor cells were cultured in vitro, which resulted in sphere formation. Sphere-forming cells were used for gene expression analysis. Immortalized primary astroglia (AG) was analzyed for comparison. mRNA profiles of astroglia (AG), olfactory bulb (OB) tumor cells and brainstem (BS) tumor cells were generated by deep sequencing, in triplicate, using Illumina HiSeq3000.