RNAseq of arabidopsis plants at different cadmium concentrations

We performed transcriptome-wide analyses (RNAseq) of Arabidopsis thaliana plants at different cadmium concentrations to evaluate its physiological effect. Seeds of Arabidopsis thaliana (L.) Heynh ecotype Columbia-0 (Col-0) and drm1 drm2 cmt3.11 (ddc) mutant in Columbia-0 (Col-0) background were grown in half-strength MS medium 1 percent sucrose and stratified at 4 degrees for 48 h. The plated seeds were then exposed at 21 degrees under long-day conditions with white light and 50 percent relative humidity until 21 days after germination (DAG) and used as control conditions (Ctrl). For Cd treatment, the medium was supplemented with 25 mM and 50 mM Cd by directly adding an aliquot of CdCl2 water stock solution (100 mM) to medium immediately before placing it in the Petri dishes.