Proteins detected by mass spectrometry after proximity biotinylation in HEK293T cells by split-APEX with tagged SRSF1 and splicing helicases.

Scaffold files following mass spectrometry of proteins isolated on streptavidin beads following proximity biotinylation. This was done using (1 file) full-length APEX2 fused to the N-terminus of SRSF1 and (1 file) split-APEX, with complementary portions of APEX2 fused to the N-terminus of SRSF1 and to the N-terminus of helicases involved in pre-mRNA splicing. Experiments were done in triplicate.