Gene expression profiling of Tg(14xUAS:GFP-EWSR1-ERG) x Et(E1b:Gal4-VP16)s1101t, Tg(UAS:Kaede) s1999t zebrafish embryos at 3 days post fertilization. Danio rerio

We use next generation sequencing of embryos with neuronally expressed EWSR1-ERG at 3 dpf to investigate overlap between these embryos' regulated gene set and that of other Ewing sarcoma models Overall design: This deep sequence study was designed to determine the gene expression profile regulated by EWSR1-ERG. Samples were obtained at 3 days post fetilisation from Tg(14xUAS:GFP-EWSR1-ERG) x Et(E1b:Gal4-VP16)s1101t, Tg(UAS:Kaede) s1999tRNA embryos expressing EWSR1-ERG neuronally, or wild-type siblings. RNA was isolated from 15 embryos per sample using the RNeasy Micro Kit (Qiagen).