Gene expression in pachytene spermatocytes and round spermatids depleted of HDAC3

This study aims to explore the transcriptomic changes in Stra8-cre/Hdac3fl/- mice at meiotic and postmeiotic stages. Pachytene spermatocytes and round spermatids were isolated from testes of Hdac3fl/+ and Stra8-cre/Hdac3fl/- mice at 7-8 week-old through STA-PUT method. Cells from 6 WT mice or 10 Stra8-cre/Hdac3fl/- mice were pooled for one biological replicate. RNA-seq libraries were prepared in biological triplicates. Total RNA was extracted from pachytene spermatocytes and round spermatids isolated from wild-type and Stra8-cre/Hdac3fl/- using TRIzol reagent. For each group, we have biological triplicates. Strand-specific libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation kit (Illumina) according to the manufacturer's instructions.