Preincubation of compound 1 with GTPases had no effects on EC50s.EC50
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The incubation time was 2 h before the addition of BODIPY-FL GTP. The experiments with and without incubation were carried out in parallel on the same day, in duplicates and repeated. Cdc42_1 was purified in house while Cdc42_2 was purchased from Cytoskeleton. Rab7_1 and Rab7_2 were from two separate preparations purified in house. Preincubation of compound 1 with GTPases had no effects on EC50s.EC50
创建时间：
2015-08-06
