Gut microbiota composition and functionality are associated with REM sleep duration and continuous glucose levels

Context: Sleep disruption is associated with poorer glucose metabolic control and altered gut microbiota in animal models. Objective: We aimed to evaluate the possible links among REM sleep duration, continuous glucose levels, and gut microbiota composition. Design: This is an observational, prospective, real-life, cross-sectional case-control study. Setting: Tertiary Hospital recruiting healthy volunteers. Patients or Other Participants: One-hundred and eighteen (60 with obesity), middle-aged (39.1-54.8) subjects. Intervention(s): Glucose variability and REM sleep duration were assessed by 10-day continuous glucose monitoring (CGM) (Dexcom G6®) and wrist-actigraphy (Fitbit Charge 3®), respectively Main Outcome Measure(s):. Glucose variability was evaluated through standard deviation (SD), coefficient of variation (CV), and interquartile range (IQR). The percentage of time in range (% TIR), 126-139 mg/dL (TIR2), and 140-199 mg/dL (TIR3) were calculated. Shotgun metagenomics sequencing ..., , , # REM sleep, glycemia and gut microbiota\_Supplemental material ## Description of the data and file structure Files: Supplemental Material.xls Contains the following spreadsheets: * Supplemental Table 1 (ST1). Bivariate analyses of REM sleep duration and clinical and glycemic outcomes. * Supplemental Table 2 (ST2). Glycemic outcomes and sleep monitoring over a 10-day period excluding subjects with OSAS. * Supplemental Table 3 (ST3). Bivariate analyses of REM sleep and clinical and glycemic outcomes excluding subjects with OSAS. * Supplemental Table 4 (ST4). Multivariate regression analysis to predict the % TIR3 and glucose variability (SD) excluding subjects with OSAS (n=101). * Supplemental Table 5a (ST5a). Differential bacterial abundance (pFDR<0.1) associated with REM sleep duration as calculated by ANCOM-BC from shotgun metagenomic sequencing adjusting for age, BMI and sex. * Supplemental Table 5b (ST5b). Differential bacterial abundance (pFDR<0.1) associated...