aDifference in PCR cycle time (ΔCt) determined by subtracting the Ct value of the PCR reaction using primers ‘7/8F’ and ‘9R’ from the Ct value of the PCR reaction using primers ‘7/9F’ and ‘9R’ (see Fi
The Percentages of Y93 Wild and Y93H Mutant Oligonucleosides Measured by Real-Time PCR Using Cycling Probe Mixtures Following Adjustment to Different Percent Volumes of Both Oligonucleosides.
