RNA-Seq on common bacterial pathogens in the presence vs absence of a variety of antibiotics. Two susceptible and two resistant isolates were exposed to antibiotics in laboratory culture, total RNA wa
A major challenge for RNA-seq analysis of gene expression is to achieve sufficient coverage of informative non-ribosomal transcripts. In eukaryotic samples, this is typically achieved by selective oli
These experiments were designed to quantify depletion of rRNA sequencing reads from bacterial RNA-seq libraries and verify that mRNA sequencing reads were not altered. Specifically, we tested an rRNA
