Factor Xa and factor IIa inhibitors differentially regulate inflammation in myocardial ischemia reperfusion injury [20-0174 RNA-seq]

While thrombin is the key protease in regard to thrombus formation, other coagulation proteases, such as fXa or activated protein C (aPC), independently modulate intracellular signaling via partially disjunct receptors. Hence, we postulate that inhibition of fXa or fIIa conveys different effects in myocardial ischemia-reperfusion injury (IRI) in regard to inflammation despite comparable anticoagulant efficacy. Overall design: Wild-type mice (C57BL/6, age 8-10 weeks) received fXai (Rivaroxaban; 3 mg/kg) or fIIai (Dabigatran; 10 mg/kg) for 7 days. Mice were sacrificed at the end of study period and heart samples including infarcted part were isolated and RNAseq was performed.