Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [ILC2_after_irr]

The goal of this study was to understand the role of thymic innate lymphoid cells (ILC2) in thymic regeneration following irradiation-induced acute involution, and determine whether this is activated by the alarmin IL-25. For this purpose we treated WT or Il25 KO mice with sublethal irradiation and/or recombinant IL-25, sorted ILC2 cells from their thymi 3 days after treatment when the thymus begins its recovery, and performed bulk RNA Seq. Young WT and Il25 KO mice were treated with sublethal irradiation, rIL-25, or both, 3 days before sacrifice, and then ILC2 were isolated and mRNA profiles were generated by deep sequencing using the MARSseq method. 3-4 replicates were used. For treated WT mice, the control was untreated age-matched WT mice, and for Il25 KO irradiated mice, the control was treated WT age-matched mice.