YjcC effect on the mouse virulence.
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The tested bacterial strains CG43S3, CG43S3ΔyjcC,CG43S3ΔyjcC[pJR1] and CG43S3ΔyjcC[pJR2] were cultured in LB medium at 37°C for overnight.Five mice of a group were injected intraperitoneally with bacteria resuspended in 0.2 ml of saline in 10-fold steps graded doses. The LD50, based on the number of survivors after one week, were calculated by the method of Reed and Muench (53) and expressed as CFU.
创建时间：
2015-12-02
