KSR1 and genotoxic agents in MCF7 (SILAC-MS)

We use KSR1 overexpression and exposure to doxorubin or etoposide in MCF7 cells to reveal a comprehensive repertoire of thousands of proteins identified in each dataset and compare the unique proteomic profile as well as functional connections modulated by KSR1 after doxorubicin (Doxo-KSR1) or etoposide (Etop-KSR1) stimulus. From the up-regulated top hits, several proteins, including STAT1, ISG15 and TAP1 were also found to be positively associated with KSR1 expression in patient samples. Moreover, high KSR1 expression, as well as high abundance of these proteins, is correlated with better survival in breast cancer patients who underwent chemotherapy. Our data exemplify a broad functional network conferred by KSR1 with genotoxic agents and highlight its implication in predicting chemotherapy response in breast cancer.