Deep-sequencing of small RNAs from barley shoot and root growing in low-Pi and control condition.

RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. We used a modified method that allows for enrichment of small RNAs. Libraries were prepared using TruSeq Small RNA Library Preparation Kit (Illumina), followed by sequencing of NGS libraries. Total RNA isolation, libraries preparation, single-reads (50 cycles) sequencing, Illumina technology, HiSeq 4000, sequencing performed at Fasteris SA (Switzerland).