Pharmacologically enhanced regulatory hematopoietic stem cells (HSC.Regs) reverts experimental autoimmune diabetes (Mouse)

Ex-vivo pharmacological modulation enhances the immunoregulatory and trafficking properties of HSCs which mitigated autoimmune diabetes and other autoimmune disorders. We used GeneChip microarrays to compare the whole transcriptomes of vehicle (DMSO) and dmPGE2 (10uM) + Dexamenthasone (uM) modulated NOD mouse Lin- c-Kit+ (LKs) HSPCs. Total RNA was extracted from in vitro modulated (4hr) NOD murine Lin- c-Kit+ (LKs) HSPCs. Each sample was processed with douplicate biological samples and gene expression due to ex vivo modulation was compared.