NanoSIMS files of Acidovorax sp. ST3 biofilm and biominerals obtained under denitrifying and As(III) & Fe(II)-oxidizing conditions, after 7 & 14 days of incubation, using the Cs+ beam

Data for Figures 1, 2 and S5 in paper:

"Elucidating
Heterogeneous Iron Biomineralization Patterns in a Denitrifying
As(III)-Oxidizing Bacterium: Implications for Arsenic Immobilization"
Sample preparation:

Acidovorax sp. strain ST3 cells were grown
anoxically in low phosphate medium with 13C-labelled acetate (10 mM), nitrate (10 mM), arsenite (0.5 mM) and Fe(II) (4 mM) for 7 or
14 days. Si wafers were introduced in the incubation bottles, to support surface colonization and precipitation of
biominerals. The samples were fixed with glutaraldehyde and dehydrated with
ethanol solutions, air-dried and Pt coated (10 nm).
NanoSIMS analysis details:

NanoSIMS analysis was
performed in a NanoSIMS 50L ion microprobe (CAMECA) using a Cs+ primary
ion beam at 16 keV. The beam current was between 0.131-1.435 pA with a spatial
resolution from 150-300 nm (D1 aperture= 4-2). The negative secondary ions detected simultaneously were: 12C, 13C, 12C14N, 28Si, 56Fe12C, 56Fe16O
and 75As. An ion-induced SE image was also obtained. The
regions of analysis (ROIs) were implanted with Cs+ ions to a
dose of 1x1017 ions cm-2 to remove the Pt
coating and reach steady state. The dwell time for image collection was between 2000-5000
µs px-1. The CAMECA mass resolving power (MRP) was >7000 using
ES=3 and AS=2. The image pixel sizes were 128 x 128 or 256 x 256. The field
of view of these nanoSIMS ions images was 30-60 µm.
The data was processed using the L’image
software (Larry Nittler, Carnegie Institution of Washington) and the plugin
OpenMIMS for ImageJ (www.nrims.harvard.edu).

About the files:
Three and nine nanoSIMS files are presented for days 7 and 14 of incubation, respectively. Files are presented in the .im raw nanoSIMS file format with additional complementary png images showing all the masses for each area of interest (AOI). All normalised counts and AOI details are compiled in the Excel spreadsheet.