Expression profiling of Tex15 wt, Tex15 ht and Tex15 ko germ cells

We repport the effect of Tex15 inactivation on the transcriptome of male germ cells at embryonic day 16.5 (e16.5), 18.5 (e18.5), and postnatal day 2 (d2). We find upregulation of a large number of transposable elements in Tex15-/- germ cells. RNA-seq of Tex15 wt (d2 only), Tex15+/- and Tex15-/- male germ cells at three timepoints (e16.5, e18.5 and d2) in biological triplicates (Illumina HiSeq 4000); Germ cells were FACS-sorted (Oct4-GFP) from testes of embryos or pups from Tex15+/- x Tex15+/- matings.本研究报道了Tex15基因失活对胚胎期16.5天（e16.5）、胚胎期18.5天（e18.5）以及出生后第2天（d2）雄性生殖细胞转录组的影响。研究发现，在Tex15基因敲除纯合型（Tex15-/-）的生殖细胞中，大量转座元件呈现上调表达。本数据集包含针对上述三个时间点的Tex15野生型（仅包含d2时期样本）、Tex15杂合型（Tex15+/-）与Tex15敲除纯合型（Tex15-/-）雄性生殖细胞的RNA测序（RNA-seq）数据，实验设置三次生物学重复，测序平台为Illumina HiSeq 4000。生殖细胞从Tex15+/-与Tex15+/-交配获得的胚胎或幼崽的睾丸中分离，分选过程以Oct4-GFP为标记，采用荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）完成。