Effect of the USP7 inhibitor HBX41,108 on lipopolysaccharide induced gene expression in mouse bone marrow derived macrophages.

USP7 is a deubiquitinase enzyme that removes polyubiquitin chains form a number of substrates including MDM2, p53 and NF-κB. In this study we assessed the impact of the USP7 inhibitor HBX41,108 on lipopolysaccharide (LPS) induded transcriptional responses in murine bone marrow derived macrophages. Three experimental groups were analysed. Untreated cells, cells stimulated with 100ng/ml LPS for 4 hours and cells pre-treated with 10µM HBX41,108 for 30 mins prior to LPS treatment. Two samples from independent experiments were analysed per group.