Transcriptome profiling of GLUTag cells with NaHS and GSH treatment

To investigate the effect of H2S on GLP-1 production, GLUTag cells were treated with NaHS, a quick-releasing H2S donor. GSH was used as a redox control. Overall design: Transcriptome profiling of GLUTag cells with NaHS and GSH treatment. Twelve-well cell culture plates were coated with 10 µg/mL poly-D-lysine overnight at 37°C. GLUTag cells were seeded at the density of 5 x 105 cells/well and cultured in DMEM containing 10% FBS and 1x penicillin-streptomycin solution for 48 hrs. Cells were then washed once with DPBS and incubated with 1mM NaHS or 0.33 mM GSH in DMEM for 2 hrs. Cells were harvested for RNA extraction and RNA-sequencing.