Gene expression in chicken wing flight feather regeneration. Gene expression in chicken wing flight feather regeneration

Avian feather has robust regeneration capability. Long noncoding RNAs (lncRNAs) are non-protein coding transcripts that are involved in various biological processes. We infected the regenerating feather follicles with lentivirus to over express Wnt5a or knock down two lncRNAs (lnc3501&lnc7831) separately. The regenerated follicles were dissected at 4 days after infection. We compared gene expression of infected follicles with normal T4 control follicles. Our results revealed that lncRNAs may modulate Wnt signaling. Overall design: Feather follicles were plucked to induce regeneration. Lentiviral vectors were used to overexpress (pLVX-Zsgreen) or knockdown (pLL3.7) gene expression in the feather follicle. The regenerated feather follicles from the wing flight feather were dissected under a stereo dissection microscope. Total RNAs of five pooled follicles were extracted by using Trizol reagent and standard protocols were followed.