Genes relevant to tissue response to cancer therapy display diurnal variation in mRNA expression in human oral mucosa. Genes relevant to tissue response to cancer therapy display diurnal variation in mRNA expression in human oral mucosa

We conducted RNA-seq assay on individual oral mucosal samples collected from 11 healthy volunteers every 4 hours (6 time points). Using a cosine-based method, we estimated the individual and average values of peak-time and amplitude for each gene. Correlations between gene expression peak-times and age was examined, adjusting for individual's sleep timing. Overall design: 11 subjects. 6 samples per subject taken every 4 in a lapse of 24 hours