Variability of 7K and 11K SomaScan Plasma Proteomics Assays

SomaScan is an aptamer-based proteomics assay designed
for the
simultaneous measurement of thousands of human proteins with a broad
range of endogenous concentrations. The 7K SomaScan assay has recently
been expanded into the new 11K version. Following up on our previous
assessment of the 7K assay, here, we expand our work on technical
replicates from donors enrolled in the Baltimore Longitudinal Study
of Aging. By generating SomaScan data from a second batch of technical
replicates in the 7K version as well as additional intra- and interplate
replicate measurements in the new 11K version using the same donor
samples, this work provides useful precision benchmarks for the SomaScan
user community. Beyond updating our previous technical assessment
of the 7K assay with increased statistics, here, we estimate interbatch
variability, assess inter- and intraplate variability in the new 11K
assay, compare the observed variability between the 7K and 11K assays
(leveraging the use of overlapping pairs of technical replicates),
and explore the potential effects of sample storage time (ranging
from 2 to 30 years) in the assays’ precision.

SomaScan是一款基于适体技术的蛋白质组学检测方法，旨在对具有广泛内源性浓度的成千上万种人类蛋白质进行同时测量。近期，7K SomaScan检测法已扩展至新的11K版本。继我们对7K检测法的先前评估之后，本研究进一步拓展了对招募于巴尔的摩纵向衰老研究（Baltimore Longitudinal Study of Aging）的捐赠者样本的技术重复数据的分析。通过生成7K版本的第二批技术重复数据以及使用相同捐赠者样本在新的11K版本中进行额外的板内和板间重复测量，本研究为SomaScan用户群体提供了有益的精确度基准。除了更新我们先前对7K检测法的统计学技术评估之外，本研究还估算批间差异，评估新11K检测法的板间和板内差异，比较7K和11K检测法观察到的差异（利用重叠技术重复对），并探讨样本储存时间（2至30年不等）对检测法精确度的潜在影响。