DMXL1 interaction proteomics from U2OS cells

To identify protein interactions with DMXL1, U2OS cells were engineered to either stably over-express DMXL1 tagged with mNeonGreen (mNG) or express HA-dTAG-tagged DMXL1 from the endogenous locus. Tagged DMXL1 was pulled-down by immunoprecipitation (IP) and IP samples were processed for TMT-MS analysis. As negative control, pull-down was also performed from parental U2OS cells. Each condition was performed in triplicate.