Reversible effects of glial or neuronal Nkt knockdown on adult locomotor activity

Figure . Reversibility of Nkt knockdown on adult locomotor activity. A) Flies have increased activity when temperature is raised from 23°C to 30°C, using the TARGET temperature-sensitive expression system. Flies were reared at 23°C. B) Knockdown flies expressing GAL80ts had high nocturnal activity at 30°C but activity in the normal range when transferred to 23°C. TR, tub-GAL80; repo-GAL4; TR>U-Nkt.IR, tub-GAL80; repo-GAL4>U-Nkt.IR; TS, tub-GAL80; nSyb-GAL4; TS>U-Nkt.IR, tub-GAL80; nSyb-GAL4>U-Nkt.IR; U-Nkt.IR, UAS-Nkt.IR. Error bars represent SEM. **p < 0.01 compared to GAL4 or UAS controls.

图 . 成年果蝇Nkt基因敲低对运动活动可逆性的研究。A) 使用TARGET温度敏感表达系统，将温度从23°C升至30°C时，果蝇的活动量增加。果蝇在23°C下进行饲养。B) 表达GAL80ts的敲低果蝇在30°C时夜间活动量较高，但当转移至23°C时，其活动量处于正常范围。TR，tub-GAL80；repo-GAL4；TR>U-Nkt.IR，tub-GAL80；repo-GAL4>U-Nkt.IR；TS，tub-GAL80；nSyb-GAL4；TS>U-Nkt.IR，tub-GAL80；nSyb-GAL4>U-Nkt.IR；U-Nkt.IR，UAS-Nkt.IR。误差棒表示标准误（SEM）。与GAL4或UAS对照组相比，**p < 0.01。