Gene expression signature connects zfp-1, rde-4 and the insulin-signaling pathway.

(A) pdk-1 mRNA levels and mRNA levels of downstream targets repressed by insulin signaling as measured by real time RT-qPCR in the indicated mutants (L4 stage animals) and normalized to wild type. Results of three biological replicas are shown; error bars represent Standard deviation. (B) zfp-1 and sod-3 mRNA levels measured by real time RT-qPCR in daf-2; daf-16 double mutant L4 worms and normalized to daf-2 mutant background, results of two biological replicas are shown. (C) Insulin-signaling pathway in C. elegans modified according to results shown in (A, B) and Figure 1.