Table 3. Liver uptake of BK- and JC-VLPs from blood takes place in NPCs.
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The mice were injected intravenously with 125I-labelled VLPs, approximately 0.5 µg per animal, and euthanized after 10 min. Liver cells were then isolated by in situ perfusion with collagenase, and separated by low-speed differential centrifugation and subsequent density centrifugation on a Percoll gradient [47]. Radioactivity (counts per minute) was measured in the purified hepatocyte fraction, and in the NPC fraction enriched in LSECs and Kupffer cells.Table 3. Liver uptake of BK- and JC-VLPs from blood takes place in NPCs.
创建时间:
2015-12-02



