Equine grass sickness (a multiple systems neuropathy) is associated with alterations in the gastrointestinal mycobiome. Equine grass sickness and the gastrointestinal mycobiome

Equine grass sickness (EGS) is a multiple systems neuropathy of grazing horses of unknown aetiology. To identify potential causal fungi, the gastrointestinal mycobiota of EGS horses were evaluated using targeted amplicon sequencing, and compared with those of two control groups. Samples were collected post mortem from up to 5 sites in the gastrointestinal tracts of EGS horses and from control horses. Faecal samples were also collected from healthy control horses that were co-grazing pastures with EGS horses at disease onset.