Data supporting A deficiency screen identifies genomic regions critical for sperm head-tail connection

These data include images that represent the mean data point from this publication, plus the original images (data) from which the figures from the manuscript were generated. The amount of space needed to upload the data from all data points is prohibitive in this format. We will gladly provide all the data, however, upon request by anyone. We also include the excel sheets containing the HTCA and raw fertility counts.Publication AbstractThe Sperm Neck provides a stable connection between the sperm head and tail, which is critical for fertility in species with flagellated sperm. Within the Sperm Neck, the Head-Tail Coupling Apparatus (HTCA) serves as the critical link between the nucleus (head) and the axoneme (tail) via the centriole. To identify regions of the Drosophila melanogaster genome that contain genetic elements that influence HTCA formation, we undertook a two part screen using the Drosophila deficiency (Df) kit. For this screen, we utilized a sensitized genetic background that overexpresses the pericentriolar material regulatory protein Pericentrin-Like Protein (PLP). We had previously shown that PLP overexpression (PLPOE) disrupts the head-tail connection in some spermatids, but not to a degree sufficient to reduce fertility. In the first step of the screen we tested for Dfs that in combination with PLPOE cause a reduction in fertility. We ultimately identified 11 regions of the genome that resulted in an enhanced fertility defect when combined with PLPOE. In the second step of the screen we tested these Dfs for their ability to enhance the head-tail connection defect caused by PLPOE, finding 6 genomic regions. We then tested smaller Dfs to narrow the region of the genome that contained these enhancers and examined the expression patterns of the genes within these Dfs using publicly available datasets of Drosophila tissue RNAseq and Drosophila testes snRNAseq. In total, our analysis suggests that some Dfs may contain single genes that influence HTCA formation or fertility, while other Dfs appear to be genomic regions rich in testis-expressed genes that might affect the HTCA through complex, multi-gene interactions.

本数据集包括代表该出版物平均数据点的图像，以及生成手稿中图表的原始图像（数据）。由于所有数据点在此格式下所需的空间量巨大，因此上传数据的成本过高。然而，我们愿意在任何人提出请求的情况下提供所有数据。此外，我们还包含了包含HTCA和原始生育计数数据的Excel表格。研究摘要：精子颈部在精子头部与尾部之间提供了一种稳定的连接，这对于具有鞭毛精子的物种的生育能力至关重要。在精子颈部内，头部-尾部耦合装置（HTCA）通过中心体作为核（头部）与轴丝（尾部）之间的关键联系。为了确定含有影响HTCA形成的遗传元素的果蝇黑腹果蝇（Drosophila melanogaster）基因组区域，我们采用Drosophila缺陷（Df）试剂盒进行了两阶段筛选。为此筛选，我们利用了一种敏感的遗传背景，该背景过度表达围中心体物质调节蛋白Pericentrin-Like Protein（PLP）。我们先前已证明PLP过度表达（PLPOE）会破坏某些精子细胞的头部-尾部连接，但程度不足以降低生育能力。在筛选的第一步中，我们测试了与PLPOE结合会导致生育能力降低的Dfs。最终，我们确定了11个基因组区域，当与PLPOE结合时，会导致生育缺陷增强。在筛选的第二步中，我们测试了这些Dfs增强PLPOE引起的头部-尾部连接缺陷的能力，发现了6个基因组区域。然后，我们测试了更小的Dfs以缩小包含这些增强子的基因组区域，并利用公开的果蝇组织RNA测序和果蝇睾丸snRNA测序数据集检查了这些Dfs中基因的表达模式。总的来说，我们的分析表明，一些Dfs可能包含影响HTCA形成或生育能力的单个基因，而其他Dfs似乎富含睾丸表达基因的基因组区域，这些基因可能通过复杂的、多基因相互作用影响HTCA。