five

Gene expression of anti-TNF induced macrophages

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE100129
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Anti-TNF induced macrophages from MLR (6) were compared to IFNγ (6), IL-4 (6) and IL-10 (6) polarized macrophage subsets. PBMC from healthy volunteers were co-cultured in a 1:1 ratio to create a MLR. Anti-TNF (infliximab) was added on day 2 and CD14+ macrophages were isolated on day 7. Monocytes from the same donors were isolated from PBMC on day 0 using Percoll density-gradient centrifugation. Monocytes were then polarized with IFN-γ, IL-4 or IL-10 for 7 days.
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2021-07-25
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