Data from: Constitutive turnover of histone H2A.Z at yeast promoters requires the preinitiation complex

The assembly of the preinitiation complex (PIC) occurs upstream of the +1
nucleosome which, in yeast, obstructs the transcription start site and is
frequently assembled with the histone variant H2A.Z. To understand the
contribution of the transcription machinery in the disassembly of the +1
H2A.Z nucleosome, conditional mutants were used to block PIC assembly. A
quantitative ChIP-seq approach, which allows detection of global occupancy
change, was employed to measure H2A.Z occupancy. Blocking PIC assembly
resulted in promoter-specific H2A.Z accumulation, indicating that the PIC
is required to evict H2A.Z. By contrast, H2A.Z eviction was unaffected
upon depletion of INO80, a remodeler previously reported to displace
nucleosomal H2A.Z. Robust PIC-dependent H2A.Z eviction was observed at
active and infrequently transcribed genes, indicating that constitutive
H2A.Z turnover is a general phenomenon. Finally, sites with strong H2A.Z
turnover precisely mark transcript starts, providing a new metric for
identifying cryptic and alternative sites of initiation.