The effect of periodontitis - induced trained immunity on the epigenomic profile of mouse bone marrow LSK and GMP cells

Peripheral inflammation affects hematopoietic stem and progenitor cells (HSPCs) in the bone marrow and induce myeloid lineage skewing of the progenitor cells. In this study, we performed single cell ATAC-sequencing in LSK (Lin—Sca-1+cKit+ ) and GMP (Lin—c-Kit+Sca1—CD16/32+CD34+) cells to determine the impact of ligature-induced periodontitis (LIP) on the epigenomic profile of these BM cells. C57BL/6 mice were trained by subjecting them to LIP for 21 days followed by ligature removal (to enable inflammation resolution) for 14 days (21dL/14dR, L) or were not ligated during the entire period (NL, N). Hematopoietic progenitors (LSKs; Lin-cKit+Sca1+) and granulocyte-macrophage progenitors (GMP; Lin-cKit+Sca1-CD16/32+CD34+) in mice bone marrow were sorted by FACS for single cell ATAC-seq analysis of epigenomic profile.