Impaired age-associated mitochondrial translation is mitigated by exercise and PGC-1α

To investigate age-related changes in muscle mitochondrial proteostasis, as well as the effect of higher and lower levels of peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) in this paradigm, we isolated RNA from M. gastrocnemius muscle tissue of muscle-specific PGC-1α gain- and loss-of-function mouse models, at the ages of three and twenty four months. We performed gene expression profiling analysis using RNA-seq, which was performed with 5-6 biological replicates for each condition.