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Single cell RNAseq analysis of undifferentiated skeletal cells in the murine limb bud

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A single cell transcriptional profile of undifferentiated skeletal cells in the murine limb bud marked by Prrx1-cre at E11.5 tdTomato+ cells were FACS-isolated from embryonic day 11.5 (E11.5) hind limbs of Prrx1-cre; R26R-tdTomato+ mice. 15,000 cells were loaded onto 10X Genomic Chromium Single Cell Expression Solution version 3 platform. cDNA was sequenced by Illumina NovaSeq 6000

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