Tyrosine phosphorylation of CARM1 promotes its enzymatic activity and alters its target specificity. Tyrosine phosphorylation of CARM1 promotes its enzymatic activity and alters its target specificity

CARM1 Y149F, Y334F or Y149F/Y334F mutants have decreased activtiy to dimethylate RUNX1. Overall design: CARM1 Y149F, Y334F or Y149F/Y334F mutations were introduced into HEL cells. ChIP-seq analyses for RUNX1 and RUNX1 R319me2a were performed in wild type and CAMR1 mutant HEL cells.