The mutants were designed to interfere with inter-domain interactions. The activity was determined according to insulin reduction as described in the text. The activity of WT PDI was taken as 100%. Da
The mutants were designed to interfere with inter-domain interactions. The activity was determined according to insulin reduction as described in the text. The activity of WT PDI was taken as 100%. Da
The inhibitory activities of SdPI and its mutants on the hydrolysis of synthetic chromogenic substrates by trypsin were assayed in 100 mM Tris-HCl (pH 8.0), containing 10 mM CaCl2 at 25°C. Trypsin was
