RNA-seq Analysis of Tgfbr2f/f and Tgfbr2HKO Mouse Liver Transcriptomes. RNA-seq Analysis of Tgfbr2f/f and Tgfbr2HKO Mouse Liver Transcriptomes

Purpose: To identify the alterations of genes transcriptional profile of livers obtained from Tgfbr2f/f mice and Tgfbr2HKO mice treated with CCl4. Methods: we generated hepatocyte-specific Tgfbr2 knockout (Tgfbr2HKO) mice by AAV8-TBG-Cre injection via the tail vein of Tgfbr2f/f. Tgfbr2f/f mice and Tgfbr2HKO were intraperitoneally injected with 0.25 ml/kg CCl4 (diluted 1:3 (v/v) in olive oil) twice a week for 4 weeks to induce liver fibrosis. Overall design: Comparative gene expression profiling analysis of RNA-seq data of 12-week old Tgfbr2f/f mice and Tgfbr2HKO mice treated with CCl4.