Quantitative Analysis of Wild Type,Ctnnb1 deltaEx3 and Ctnnb1 deltaEx2-6 Spinal Cord Transcriptomes. Quantitative Analysis of Wild Type,Ctnnb1 deltaEx3 and Ctnnb1 deltaEx2-6 Spinal Cord Transcriptomes

The goals of this study are to analyze NGS-derived spinal cord transcriptome profiling (RNA-seq) of wild type (WT), Nestin-Cre mediated Ctnnb1 Ex3 deletion (gain of function, dE3) and Ex2-6 deletion (loss of function, cKO) at embryonic day 13.5 (E13.5). Spinal cord RNA profiles were generated by deep sequencing, using Illumina Hiseq. The sequence reads that passed quality filters were analyzed at the transcript isoform level with HISAT2 and RSeQC. Overall design: Spinal cord RNA profiles of E13.5 wild type (WT), Ctnnb1-dE3 and Ctnnb1-cKO mice were generated by deep sequencing, using Illumina Hiseq 2500.