Microarray of CD11b+CD11c–Ly6Chi inflammatory monocytes in tumor tissue and spleens from Th9 treated mice. Microarray of CD11b+CD11c–Ly6Chi inflammatory monocytes in tumor tissue and spleens from Th9 treated mice

We recently reported that tumor-specific Th9 cells are less susceptible to exhaustion, fully cytolytic to tumor cells, and possess long-term persistence capacity because of their unique hyperproliferative T cell feature. However, these observations only revealed how tumor-specific Th9 cells eradicate antigen-positive tumor cells. Given the unmet need to develop effective ACT strategies with tumor-specific Th9 cells for solid tumors with the heterogeneity in antigen expression, the CD11b+CD11c–Ly6Chi inflammatory monocytes were flow-sorted from Th9 treated mice, and been further analyzed for microarray. Overall design: Mice were treated with Th9 cells, and 10 days after Th9 transfer, tumor tissue and spleens were harvested and dissociated for cell sorting. CD11b+CD11c–Ly6Chi inflammatory monocytes were flow-sorted. Total RNA was extracted with the RNeasy Mini kit (Qiagen)