Gene expression profile at single cell level of mononuclear cells from skeletal muscle (gastrocnemius) of non-PAD controls and PAD patients

The molecular and cellular mechanisms of skeletal muscle from peripheral artery disease (PAD) patients are not completely understood. We used single cell RNA sequencing (scRNA-seq) to characterize the skeletal muscle diversity between control and PAD patients. Live mononuclear cells (Calcein+) from human skeletal muscle were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using scRNAseq.