Oophaga sylvatica decahydroquinoline quantification

Shifts in microbiome community composition can have large effects on host health. It is therefore important to understand how perturbations, like those caused by the introduction of exogenous chemicals, modulate microbiome community composition. In poison frogs within the family Dendrobatidae, the skin microbiome is exposed to the alkaloids that the frogs sequester from their diet and use for defense. Given the demonstrated antimicrobial effects of these poison frog alkaloids, these compounds may be structuring the skin microbial community. To test this, we first characterized microbial communities from chemically defended and closely related non-defended frogs from Ecuador. Then we conducted a laboratory experiment to monitor the effect of the alkaloid decahydroquinoline (DHQ) on the microbiome of a single frog species. In both the field and lab experiments, we found that alkaloid-exposed microbiomes are more species rich and phylogenetically diverse, with an increase in rare taxa. To ..., , , # Oophaga sylvatica decahydroquinoline quantification

[https://doi.org/10.5061/dryad.gxd2547t1](https://doi.org/10.5061/dryad.gxd2547t1)

This Liquid Chromatography Mass Spectrometry (LCMS) data was generated in a controlled feeding experiment to test for the role of the alkaloid decahydroquinoline (DHQ) in shaping the skin microbiome of the frogs Oophaga sylvatica and Allobates femoralis. The quantity of DHQ was measured at the end of the experiment. Files are raw data from a Waters Acquity UPLC and Thermo Exploris 240 BioPharma orbitrap mass spectrometer. Files include mass to charge ratios, retention times, and intensities. Data files can also be opened with various open source software, including OpenChrom ([https://www.openchrom.net/](https://www.openchrom.net/)). 

Other data related to this manuscript can be found in the following locations:

[https://doi.org/10.5061/dryad.5qfttdzd5](https://doi.org/10.5061/dryad.5qfttdzd5), Wild dendrobatid frog microbiomes - bacteria
[https:/...