Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [S2]

Heat shock (HS) causes dramatic and rapid changes in transcription of thousands of genes in metazoans. We have used insitu Hi-C in Drosophila S2 and human K562 cells to determine the effects of HS upon chromatin conformation. Our study shows that topologically associated domains (TADs), compartments and distal regulatory interactions remain unchanged upon HS. Overall design: Investigate changes in chromatin conformation upon acute HS in Drosophila S2 and human K562 cells by insitu Hi-C. Two biological replicates were done for Non heat shock (NHS) or HS conditions in each organism.