Trypanosome Isolates and Amplification Results

JE samples are from Uganda and were processed using Sigma Genomic DNA extraction kit, followed by precipitation with 3 M sodium acetate and absolute alcohol. TMRS samples were from Tanzania and the DNA was prepared using Qiagen DNA extraction kit.aSubgenus Trypanozoon PCR test [7].bT. b. rhodesiense PCR test [3].cT. b. gambiense PCR test [17].dSource: Wendy Gibson, University of Bristol, UK.eThe samples were processed using the saponin lysis method [5].fDNA prepared directly from human blood.gDNA prepared directly from human CSF.hSource: Philippe Büscher, Institute of Tropical Medicine Antwerp, Belgium. DNA prepared with Qiagen DNA extraction kit.nd, not done in this study (identification reported [17]).