Aspergillus nidulans wild type and chimaera cell wall carbohydratesa, antifungal drug sensitivityac, and relative UGM activityb.

a Absorbance at 405 nm was recorded using ELISA reader, Results are presented as an index of OD values with respect to wild type. Cell walls were isolated from A. nidulans strains after 48 h growth. Index sensitivity values are for each drug/strain combination compared to wild type. Index values > 1.0 were more sensitive than wild type. Index values that differed by > 0.2 were based on data that were significantly different.b Relative UGM activity in vitro with respect to wild type, derived from data in (33) and this study for F66A. nd, not determined; na, not available (low protein expression); bd, below detection.c Caspofungin, Casp; Itraconazole, Itra; Calcofluor White, CFW. See Materials and Methods for drug dosage and medium formulation. Drug sensitivity was measured using a disc diffusion assay as described in methods (Figure S3 in File S1). For Caspofungin, sensitivity was the radius (mm) of the clear zone with no visible growth. Mean ± SE of two measurements for each of four biological replicates were used for statistical analysis (not shown).