The effect of maltose on MICAmp and protein expression.
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aProtein expressed from plasmid pDIMC8 in RH22 cells.bMICAmp was determined in triplicate. For proteins with a MICAmp range, this indicates the range from the three experiments (e.g. Ph5 was found to have a MICAmp in the absence of maltose of 16 µg/ml in some trials and 32 µg/ml in other trials).cAccumulation ratio determined from quantitative image analysis of western blots probed using polyclonal anti-BLA antibodies (except for MBP which was probed with anti-MBP antibodies).d(With maltose)/(without maltose).eStandard deviation calculated from three independent experiments.fNo pDIMC8 plasmid was present. The protein monitored by western blot was chromosomally encoded MBP.
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2015-12-02



