Inhibition of SEAM 3 binding by Neu-containing PSA oligosaccharides.

aThe lower limit of detection is ∼10% Neu in the resorcinol assay.bConcentration of inhibitor required to inhibit 50% of binding of SEAM 3 to the nominal N-Pr MBPS antigen by ELISA. For comparison, the nominal N-Pr MBPS antigen (DP>30, 16% Neu) used to produce SEAM 3 had an IC50 value of 0.01 µg/ml. The concentrations of purified SEAM 3 used in the ELISA were 0.07 µg/ml.