GNPS Metabolite diversity in two yacon cultivars

Smallanthus sonchifolius plants from two different cultivars were grown on a trial field of the University of Hohenheim (Stuttgart, Germany) until they reached the adult stage. Fifty milligrams (fresh weight) of different organs including the leaves (from three different ages), stems, roots and tubers were extracted with 1 mL of ethanol 70% and analyzed by UHPLC-UV-HRMS/MS (Q-Exactive Plus). Chromatographic separation was performed under the following gradient program: 0-15 min, 3-20% B; 15-40 min, 20-95% B; 40-43 min, 95-3% B; 43-45 min, 3% B using an Acquity CSH C18 column (1.7 um, 150 mm x 2.1 mm, Waters, Ireland), (A) water (0.2% formic acid) and (B) acetonitrile (0.2% formic acid) as mobile phase at a flow rate of 400 uL/min. UV detection was performed between 190 and 400 nm