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Phylogeny of host plant species

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DataONE2018-01-23 更新2024-06-25 收录
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Phylogenetic tree of host plant species. We downloaded accessions of 3 genes (2 plastid and 1 nuclear) for each of our plant species from GenBank: ribulose-bisphosphate carboxylase (rbcL); maturase K (matK); and internal transcribed spacer (ITS) adjacent to the 5.8S ribosomal RNA gene. We aligned sequences in MEGA v. 6.0 using MUSCLE with default parameters, followed by manually checking alignments. We used BEAST v. 2.1.3 to build a Bayesian phylogenetic tree. For each locus we implemented a standard general time-reversible model (GTR + I + Γ) and an uncorrelated lognormal clock (UCLN) to determine the rate of nucleotide change.
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2018-01-23
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