The biphasic Fusarium graminearum transcriptome during symptomless and symptomatic wheat infection. Fusarium graminearum

The analysis of the Fusarium graminearum transcriptome during symptomless and symptomatic infections of wheat floral tissue at 7 days post infection, to identify the correct spatio-temperol regulation of multiple virulence strategies. Overall design: Fusarium graminearum PH-1 was inoculated into the floral cavity of the two florets, of the two spikelets, in the middle of the wheat head. At 7 days post infection the inoculated spikelets and the 7 sequential rachis internodes below the inoculated spikelet were collected. The individual wheat tissues were pooled for ten wheat heads to form a single biological replicate. Non-infected wheat spikelets and rachis was collected as a negative control. Three biological replicate were collected per treatment.